Real-space images of soft matter with molecular and sub-molecular resolution could provide valuable insights into the relationship between morphology and functionality of such materials, but their acquisition is problematic due to perceived limitations in atomic force microscopy (AFM).

Here we demonstrate that using a higher eigenmode imaging (HEMI) approach could provide a simple and versatile route to acquiring ultra-high resolution images with conventional AFM probes both in air and liquids. HEMI is based on exciting 2nd, 3rd or higher resonance modes of standard cantilevers. Using higher frequency greatly improves both resolving capabilities and acquisition speed as well. During this webinar, we will present a quick overview of various materials/samples that were successfully imaged down to a single molecule level using this approach.