|联系我们  Global

Advances in Atomic Force Microscopy: Nanomechanical Characterization by using PinPoint™ AFM

Banner Final version

The Institute Jacques Monod, founded jointly by the CNRS and the University Paris Diderot, and Park Systems are pleased to invite you to a workshop on Advances in Atomic Force Microscopy: Nanomechanical Characterization by using PinPoint™ AFM, which will take place on October, 10 at the “Cell Adhesion and Mechanics” Group, Institute Jacques Monod.

Date: Thursday, 10 October, 2019

Time: 10.00am - 16.00pm

Place: Institute Jacques Monod, “Cell Adhesion and Mechanics” Group, 15 Rue Helene Brion, Batiment Buffon, 404B, Paris Franc

 

Program 

  • 10:00 Welcome
  • 10:15 Introduction
  • 10:30 Talk: “Advances in Atomic Force Microscopy: Nanomechanical Characterization by using PinPoint™ AFM”/ Andrea Carreta, Application Scientist, Park Systems Europe
  • 11:30 - 13:00 Live Measurements
  • 13:00 Lunch
  • 14:00 - 16:00 Live Measurements
  • 16:00 End Discussion/Wrap up

 

Abstract

Nanomechanical imaging of collagen fibrils via PinPointTM atomic force microscopy

Andrea Cerreta,1 Mina Hong,2 Gerald Pascual,2 Byong Kim,2 Keibock Lee2
1. Park SystemsEurope, Mannheim, Germany
2. Park Systems, Inc., Santa Clara, USA

Imaging the distribution of local mechanical properties on the nanoscale can significantly advance research on exciting soft matter materials relevant for biological systems (1,2) or optoelectronic applications (3). Force-distance measurements with atomic force microscopy (AFM) measure adhesion and elasticity by pressing a nanometer-sized tip on the end of a cantilever onto the surface. However, the correlation of mechanical data from force-distance measurements to the local sample morphology additionally requires topographic sample information. PinPointTM nanomechanical imaging with Park Systems AFMs simultaneously acquires topographic and force-distance data within short amounts of time. Therefore, PinPointTM AFM locally resolves the distribution of adhesion and elasticity.
Here, we characterized the mechanical properties of collagen fibrils, a structural protein occurring in ligaments and skin. As such, the study of collagen’s mechanical properties can reveal new insights on the elasticity of skin and aging processes. Via PinPointTM AFM we imaged fibrils with diameters ranging from 60 to 600 nm and calculated an average elastic modulus of 1.9 GPa in air. Upon immersion of the collagen in liquid, we observed the softening of the fibrils to 4-12 MPa, agreeing with previous findings (4). This study illustrates the additional value of PinPointTM AFM for the investigation mechanical properties in bioloical samples.

1. Perni, S., Preedy, E. C., Landini, P. & Prokopovich, P. Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli. Langmuir 32, 7965–7974 (2016).
2. Bastounis, E. E., Yeh, Y.-T. & Theriot, J. A. Matrix stiffness modulates infection of endothelial cells by Listeria monocytogenes via expression of cell surface vimentin. Mol. Biol. Cell 29, 1571–1589 (2018).
3. Kim, S. et al. Origin of macroscopic adhesion in organic light-emitting diodes analyzed at different length scales. Sci. Rep. 8, 6391 (2018).
4. Grant, C. A., Brockwell, D. J., Radford, S. E. & Thomson, N. H. Tuning the Elastic Modulus of Hydrated Collagen Fibrils. Biophys. J. 97, 2985–2992 (2009).

Registration and contact: 该Email地址已收到反垃圾邮件插件保护。要显示它您需要在浏览器中启用JavaScript。 | www.parksystems.com

 

 


About Park Systems

 

Park Systems enables researchers and engineers around the globe to contribute to impactful science and technological development that helps the humanity to grow and improve life standards. Thanks to the high-level technological know-how and 100% commitment, the professionals at Park provide to and with their customers innovative application solutions for material and life science disciplines. With Park’s high-performance scientific instruments for research and industrial communities we help to explore new phenomena in chemistry, materials, physics, life sciences, semiconductor and data storage industries. Cutting-edge AFM automation and the highest data accuracy enables to become more efficient, more accurate and more productive at your work.

Visit www.parksystems.com for more information.

 

 

Park Workshops | Park Atomic Force Microscope